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626 cell culture 627 human melanoma cell line mda mb 435s  (ATCC)


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    ATCC 626 cell culture 627 human melanoma cell line mda mb 435s
    626 Cell Culture 627 Human Melanoma Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 974 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mda+mb+435s+cell+lines/MDA-MB-435S/10__1016_slash_j__isci__2026__115846-292-0-24
    Average 96 stars, based on 974 article reviews
    626 cell culture 627 human melanoma cell line mda mb 435s - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Selective targeting capability acquired with a protein corona adsorbed on the surface of 1,2-dioleoyl-3-trimethylammonium propane/DNA nanoparticles.
    Article Snippet: A possible turning point in drug delivery has been recently reached: the protein shell, which covers nanocarriers in vivo, can be used for targeting.. Here, we show that nanoparticles can acquire a selective targeting capability with a protein corona adsorbed on the surface.. We demonstrate that lipid particles made of 1,2-dioleoyl-3trimethylammonium propane (DOTAP) and DNA, upon interaction with human plasma components, spontaneously become coated with vitronectin that promotes efficient uptake in cancer cells expressing high levels of the vitronectin ανβ3 integrin receptor.

    Article Title: Synthesis and characterization of 64 Cu-labeled Geldanamycin derivative for imaging HSP90 expression in breast cancer.
    Article Snippet: Heat shock protein 90 (HSP90) plays a crucial role in cancer cell growth and metastasis by stabilizing overexpressed signaling proteins.. Inhibiting HSP90 has emerged as a promising anti-cancer strategy.. In this study, we aimed to develop and characterize a HSP90-targeted molecular imaging probe, [64Cu]Cu-DOTA-BDA-GM, based on a specific HSP90 inhibitor, geldanamycin (GM), for PET imaging of cancers.

    Article Title: Deoxyribonucleic Acid Profiling Analysis of 40 Human Thyroid Cancer Cell Lines Reveals Cross-Contamination Resulting in Cell Line Redundancy and Misidentification
    Article Snippet: .. We analyzed the BRAF V600E mutational status in the MDA-MB-435S cells from the ATCC and found that the MDA-MB-435S cell lines express a heterozygous BRAF V600E mutation (data not shown). ..

    Article Title: Cell-specific regulation of proliferation by Ano1/TMEM16A in breast cancer with different ER, PR, and HER2 status
    Article Snippet: .. The MCF7 and MDA-MB-435S cell lines were obtained from the American Type Culture Collection (ATCC, USA). .. The cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen, USA) containing 10% fetal bovine serum (HyClone, USA), 100 U/ml penicillin, and 100 mg/ml streptomycin in 5% CO 2 in a humidified incubator at 37 °C.

    Article Title: Selective killing of cancer cells based on translational control of a suicide gene.
    Article Snippet: .. The MM2MT, MM3MG, MCF7, MCF-10A, MDA-MB231, and MDA-MB-435S cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA). .. All cell lines, except MCF-10A, were grown in DMEM high-glucose media (Gibco-BRL, Grand Island, NY), and supplemented with 10% fetal calf serum, nonessential amino acids (Gibco-BRL), and antibiotic– antimycotics (Gibco-BRL).

    Article Title: cDNA microarray analysis of invasive and tumorigenic phenotypes in a breast cancer model.
    Article Snippet: Hybridization was performed with the same hybridization buffer and 32P-labeled DNA probes (ResGen, Carlsbad, CA, USA) at 681C for 16 h. Signals on the washed filter were visualized by Kodak XAR-5 film autoradiography. .. SKBR-3, BT-20, BT-549, BT-474, MDA-MB-468, and MDA-MB-435S cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA), and grown per ATCC recommendations (http://www.atcc.org/). ..

    Article Title: Nucleolin overexpression in breast cancer cell sub-populations with different stem-like phenotype enables targeted intracellular delivery of synergistic drug combination.
    Article Snippet: Please cite this article as: Fonseca NA, Rodrigues AS, Rodrigues-Santos P, Alves V, Gregório AC, Valério-Fernandes Â, Gomes-da-Silva LC, Rosa MS, Moura V, Ramalho-Santos J, Simões S, Moreira JN, Nucleolin overexpression in breast cancer cell sub-populations with different stem-like phenotype enables targeted intracellular delivery of synergistic drug combination, Biomaterials (2015), doi: 10.1016/ j.biomaterials.2015.08.007.

    Cell Culture:

    Article Title: Selective targeting capability acquired with a protein corona adsorbed on the surface of 1,2-dioleoyl-3-trimethylammonium propane/DNA nanoparticles.
    Article Snippet: A possible turning point in drug delivery has been recently reached: the protein shell, which covers nanocarriers in vivo, can be used for targeting.. Here, we show that nanoparticles can acquire a selective targeting capability with a protein corona adsorbed on the surface.. We demonstrate that lipid particles made of 1,2-dioleoyl-3trimethylammonium propane (DOTAP) and DNA, upon interaction with human plasma components, spontaneously become coated with vitronectin that promotes efficient uptake in cancer cells expressing high levels of the vitronectin ανβ3 integrin receptor.

    Mutagenesis:

    Article Title: Deoxyribonucleic Acid Profiling Analysis of 40 Human Thyroid Cancer Cell Lines Reveals Cross-Contamination Resulting in Cell Line Redundancy and Misidentification
    Article Snippet: .. We analyzed the BRAF V600E mutational status in the MDA-MB-435S cells from the ATCC and found that the MDA-MB-435S cell lines express a heterozygous BRAF V600E mutation (data not shown). ..



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    MMP-9 downregulation is critical in IB-induced metastasis inhibition in human TNBC cells. ( a ) After treatment with 1 or 3 µM IB, wound healing scratch assays were performed using TNBC cells. Cell migration was monitored under a phase-contrast microscope for 24 h: Bar, 50 μm. Data are shown as the mean ± SD. * p < 0.05, *** p < 0.001 vs. 24 h untreated control. ( b ) Invasion assays of TNBC cells treated with 0.5 or 1 µM IB for 24 h were performed. Invading cells were stained with crystal violet and observed using a fluorescence microscope (4×). Data are presented as the mean ± SD. *** p < 0.001 vs. untreated control. ( c ) Cell extracts were prepared from cells treated with the indicated IB concentrations for 24 h. MMP-9 and MMP-2 levels were detected using Western blotting. α-tubulin was used as a loading control. ( d ) TNBC cells were treated with the indicated IB concentrations for 24 h. Cell extracts were prepared for Western blotting of mesenchymal markers. ( e ) MMP-9 overexpressing stable cells were scratched during wound healing assays. MMP-9 upregulation was confirmed using Western blotting. Cell migration was monitored for 24 h and observed under a phase-contrast microscope: Bar, 50 μm. The graphs quantitatively show the area of wound recovery. Data are presented as the mean ± SD. *** p < 0.0001 vs. IB-treated vector cells. ( f ) Invasion assays of MMP-9 overexpressing stable cell lines treated with 0.5 or 1 µM IB for 24 h were performed. Invading cells were stained with crystal violet and observed using a fluorescence microscope (4×). Data are shown as the mean ± SD. * p < 0.05, *** p < 0.001 vs. IB-treated vector cells.

    Journal: International Journal of Molecular Sciences

    Article Title: Ibulocydine Inhibits Migration and Invasion of TNBC Cells via MMP-9 Regulation

    doi: 10.3390/ijms25116123

    Figure Lengend Snippet: MMP-9 downregulation is critical in IB-induced metastasis inhibition in human TNBC cells. ( a ) After treatment with 1 or 3 µM IB, wound healing scratch assays were performed using TNBC cells. Cell migration was monitored under a phase-contrast microscope for 24 h: Bar, 50 μm. Data are shown as the mean ± SD. * p < 0.05, *** p < 0.001 vs. 24 h untreated control. ( b ) Invasion assays of TNBC cells treated with 0.5 or 1 µM IB for 24 h were performed. Invading cells were stained with crystal violet and observed using a fluorescence microscope (4×). Data are presented as the mean ± SD. *** p < 0.001 vs. untreated control. ( c ) Cell extracts were prepared from cells treated with the indicated IB concentrations for 24 h. MMP-9 and MMP-2 levels were detected using Western blotting. α-tubulin was used as a loading control. ( d ) TNBC cells were treated with the indicated IB concentrations for 24 h. Cell extracts were prepared for Western blotting of mesenchymal markers. ( e ) MMP-9 overexpressing stable cells were scratched during wound healing assays. MMP-9 upregulation was confirmed using Western blotting. Cell migration was monitored for 24 h and observed under a phase-contrast microscope: Bar, 50 μm. The graphs quantitatively show the area of wound recovery. Data are presented as the mean ± SD. *** p < 0.0001 vs. IB-treated vector cells. ( f ) Invasion assays of MMP-9 overexpressing stable cell lines treated with 0.5 or 1 µM IB for 24 h were performed. Invading cells were stained with crystal violet and observed using a fluorescence microscope (4×). Data are shown as the mean ± SD. * p < 0.05, *** p < 0.001 vs. IB-treated vector cells.

    Article Snippet: The human TNBC cell lines (Hs578T and MDA-MB-435S) were purchased from the American Tissue Culture Collection (ATCC, VA, USA).

    Techniques: Inhibition, Migration, Microscopy, Control, Staining, Fluorescence, Western Blot, Plasmid Preparation, Stable Transfection